Prevalence and patterns of Y chromosome microdeletion in infertile men with azoospermia and oligzoospermia in Northeast China

Background: In some cases infertile men showed small deletions of specific genes in the Y chromosome. It had been confirmed, these deleted genes are greatly associated with spermatogenic failure. However, the frequency and the patterns of such microdeletions among infertile men are not clearly clarified. Objective: We sought to determine the frequency and the patterns of Y chromosome microdeletions in azoospermic and oligozoospermic infertile men in Northeast China, and try to optimize the selection of sequence tagged sites (STSs) of AZF microdeletions in multiplex polymerase chain reaction (PCR). Materials and Methods: 720 azoospermic and 330 oligozoospermic infertile men, from Northeast China were included in this retrospective study during May 2008 to November 2012. Semen analysis was performed according to the World Health Organization guidelines. Y chromosome microdeletions were detected by polymerase chain reaction assays. G-banding method was used for chromosome Karyotype analysis. Chi-square tests were used to compare patterns of Y chromosome microdeletions in azoospermic and oligozoospermic patients. Results: Of 1050 infertile men, 12.95% cases had shown Y chromosome microdeletions, and 19.43% of cases showed abnormal chromosomal karyotype. Deletions in AZFc region was the most frequent 75.00%, followed by deletions in AZFb region 13.33%, AZFbc region 09.62%, and AZFa region 2.22%. All oligozoospermic patients showed presence of sY84, sY86, sY127, and sY134. Deletion of sY127 (p=0.0101) and sY157 (p=0.0043) showed significant difference between azoospermic group and oligozoospermic group. Conclusion: Deletions of sY127 may relate to azoospermia while sY84, sY86, sY127 can be ignored in AZF screening for oligozoospermic patients.


Introduction
t has been estimated that 10-15% of couples worldwide suffer from infertility, and male factor can be identified in about half of these cases (1). Genetic factors contribute about 10-15% of male infertility (2). Among these factors, Y chromosome microdeletions are the second most frequent genetic cause of male infertility after Klinefelter's syndrome (3). The sex determining Y chromosome is the smallest human chromosome consists of a short (Yp) and a long (Yq) arm. The majority of the microdeletions which cause azoospermia or oligozoospermia occur in the non-overlapping regions of the long arm, these microdeletions (compared with other structural chromosomal abnormalities) are too small to be detected by karyotyping. They can be identified using polymerase chain reaction (PCR).
The first hypothesis defined the correlation between Y chromosome deletions and male infertility was reported by Tiepolo and Zuffardi in 1976 (4). Investigation of these deletions has revealed that there are three nonoverlapping Y-chromosome regions important for normal spermatogenesis. These regions are AZFa, AZFb, and AZFc from proximal to distal Yq arm (5). Furthermore, a fourth region (AZFd) has been discovered later (6). Recently Y chromosome microdeletions are recognized as the most important genetic etiology of male infertility throughout the world in addition to close relation with recurrent abortion (7). I The incidence of Y chromosomes microdeletions was described previously, most of these previous studies described the prevalence of microdeletion involving the regions and the common patterns (AZFc, AZFbc AZFb, AZFa, and AZFabc) but no one had investigated the incidence of deletion involving the sequence tagged sites STSs (sY84, sY86, sY127, sY134, sY143, sY157, sY255, sY254 and sY152) individually.
This study aimed to determine the frequency and the patterns of Y chromosome microdeletions in infertile men with azoospermia and oligozoospermia in Northeast China, and try to reveal significant STSs among azoospermic, and oligozoospermic groups. We used multiplex PCR, to detect nine sequence tagged sites STSs (sY84, sY86, sY127, sY134, sY143, sY157, sY255, sY254, and sY152).

Patients
A retrospective study was performed to 1050 infertile men aged between 20 and 44 years old from Northeast China who attend the First, Hospital of Jilin University, were included in this study during May 2008 to November 2012. 720 suffering from azoospermia, and 330 from oligozoospermia. Patients with microdeletions associated with abnormal karyotype or obstructive azoospermia were excluded from this study. Patient's medical histories, reproductive problems, and physical examination were recorded. Semen samples were obtained in the period of 2-7 days after ejaculatory abstinence, and semen analysis was performed according to the World Health Organization guidelines (8). Peripheral blood samples were obtained and stored for cytogenetic detection. Oligozoospermia was diagnosed with a sperm count <20×10P 6 P /mL. And another 100 healthy fertile men were included as controls; every man in the control group had fathered at least one child.
This study was approved by the Reproductive Medicine Ethics Committee of First Hospital of Jilin University, and all patients signed informed consents of this study before semen analysis.

DNA extraction and PCR analysis
Peripheral blood samples were obtained from all patients and the genomic DNA was isolated using commercially-available blood DNA extraction kits (Beijing Tiangen Biotech Co., Ltd, China). Control DNA samples obtained from unrelated normal males with proven fertility and from normal females were used as positive and negative controls respectively. A sample containing all reaction components and water in place of the DNA template was used as the PCR blank control.
Based on the recommendations of the European Academy of Andrology (EAA) and the European Molecular Genetics Quality Network (EMQN), the samples were tested for classical Y chromosome microdeletions using the following sequence-tagged sites (STSs) proposed by Simoni et al: sY84, sY86, sY127, sY134, sY143, sY152, sY254, sY255, and sY157 (9). The detections of sY14 (SRY) and ZFX/ZFY were employed as internal controls. Multiplex PCR was carried out in a total volume of 30 μL using a Veriti 96-well PCR thermal cycler (Applied Biosystems, USA). The results were considered positive when a clear amplification product of the expected site was obtained.

Karyotype analysis
After culturing peripheral blood samples for 72h, lymphocyte chromosome spreads were prepared using routine methods; Karyotypes were described according to the International System for Chromosome Nomenclature (ISCN), and analyzed by G-banding (10). For each individual, a minimum of 20 metaphase cells were counted and at least five cells were analyzed.

Statistical analysis
Chi-square tests were used to compare patterns of Y chromosome microdeletions in azoospermic and oligozoospermic patients. Differences were considered to be statistically significant when p<0.05.  (Table V). All patients who showed absence of sY127 were diagnosed as azoospermic patients (Table  VI). Microdeletions involving one sequence tagged site appeared in five cases, these were sY84 (two cases), sY86 (one case), sY127 (one case) among azoospermic group, and sY143 (one case) from oligozoospermic group (Table  VI).

Out
Deletion of sY127 (p=0.0101) and sY157 (p=0.0043) showed significant difference between azoospermic group and oligozoospermic group. No significant difference was noted in the deletions involving the other sequence tagged sites (  (Table IX). No deletion was detected in AZFa region. For AZFb region, only one patient showed deletion in sY143 (1/121, 00.82%). All oligozoospermic patients showed presence of sY84, sY86, sY127 and sY134 (Table IX).

Discussion
This study dealt with the frequency and the patterns of Y chromosome microdeletions in infertile men in Northeast China. The results have shown that microdeletions were detected among 136 male patients out of 1050 infertile men, with a prevalence of 12.95% (136/1050). Compared with previous studies this findings revealed that, microdeletions are more prevalent among Northeast Chinese population as compared with Korean population where a percentage of 7.7% has been reported (11). The prevalence is similarly higher among Northeastern China population when compared with findings in Brazilian (7.5%) and Serbian population (5.4%) (3,12).
Our findings is partially similar with a relevant recent Korean study that revealed a high frequency of microdeletions in the AZFc region (87.1%) as compared to deletions in AZFbc 24.7% and AZFabc 8.9% (11). Moreover, in 2006 Song et al studied 21 cases of microdeletions among 143 infertile patients, and found that, deletions of AZFc region were at a percentage of 76.2%, of the AZFb region were at 9.5% and those of AZFa region at 4.8% (18). Furthermore, another study by Ristanovic et al reported the incidence of Y chromosome microdeletions as the following: deletions involving AZFc region estimated at about 75%, AZFa 8% and AZFbc 17% (9).
In 2009 Zhou et al reported that, deletion of the AZFc region was the most common deletions 63.8%, followed by AZFbc 19.0%, AZFabc 10.3% and AZFb or AZFa 3.4% (19). The present study concluded that, deletion of sY255 and sY254 is the most common pattern of AZF microdeletions. Hence, this conclusion is in accordance with the findings of Wu et al, who had reported that, deletions of sY255 and sY254 among Chinese population is the most common pattern of AZF microdeletions (20). In comparison between azoospermic men and oligozoospermic men, previous studies found that, the prevalence of microdeletions was higher in azoospermic than in oligozoospermic men as reported by Shi et al (9.16% and 5.56% respectively), and Zhu et al (14% and 8.2% respectively) (17,21). In our study, however, the incidence was found to be at a similar rate in the azoospermic group (101/720, 14.03%) and in the oligozoospermic group (35/330, 10.60%).
This study has revealed presence of sY84, sY86, sY127and sY134 in all oligozoospermic patients. Moreover, the present study also showed that, absence of sY84, sY86, and sY127 alone or along with other sequence tagged sites results in azoospermia. This finding is in agreement with the report of Hopps et al who noted that, men with microdeletion of the AZFa or AZFb regions of the Y chromosome have very poor amount of sperm, whereas the majority of male with AZFc deletion have sperms within the semen or testes (22). The study results showed microdeletions involving sY127 is significantly related to azoospermia. The high frequency of sY127 deletion 6.4% (16/250) among azoospermic patients and the significant difference between azoospermic group and oligozoospermic group in the deletion of sY127 may indicate that, microdeletions involving sY127 may associated with severe spermatogenic failure.
As shown in table VII, although there was no significant difference between the two groups in the deletion of sequence tagged sites sY84 and sY86, deletion involving these sequences resulting in azoospermia. This is possibly attributed to the low incidence of deletion involving these sites in general, and to the small number of samples used as compared to those employed in the present study (35 oligozoospermic deletion and 101 azoospermic deletion). The present study concluded that, microdeletions involving sY127 are related to azoospermia. Deletions of sY127 as deletions of sY84, or sY86 alone may be a high risk factor of azoospermia.